Helium Ion Microscopy of Plant Tissues and Mammalian Cells
نویسندگان
چکیده
From plants to animals, the helium ion microscope (HIM) facilitates ultrastructural investigations of biological systems at both high contrast and resolution without the need for heavy metal coatings [1]. In this study, we describe not only the advantages of HIM over the low-voltage scanning electron microscopy (LV-SEM) of plant tissues, but will also demonstrate advances in preserving sub-nanometer membrane structures of in vitro cell cultures.
منابع مشابه
Electron and Helium Ion Imaging of Arabidopsis Affected by Genetic Mutation and Thermochemical Treatment for Biofuel Applications
Lignocellulosic biomass holds unlocked potential as biofuel feedstock. By enzymatic digestion or by pyrolysis, the biopolymers that compose plant cell walls are converted into liquid biofuels at the industrial scale. One strategy to increase the efficiency and lower the cost of these conversion processes is the use of genetically modified plants that produce biomass that is more amenable cataly...
متن کاملHelium Ion Microscopy Visualizes Lipid Nanodomains in Mammalian Cells.
Cell membranes are composed of 2D bilayers of amphipathic lipids, which allow a lateral movement of the respective membrane components. These components are arranged in an inhomogeneous manner as transient micro- and nanodomains, which are believed to be crucially involved in the regulation of signal transduction pathways in mammalian cells. Because of their small size (diameter 10-200 nm), mem...
متن کاملHigh Resolution Helium Ion Scanning Microscopy of the Rat Kidney
Helium ion scanning microscopy is a novel imaging technology with the potential to provide sub-nanometer resolution images of uncoated biological tissues. So far, however, it has been used mainly in materials science applications. Here, we took advantage of helium ion microscopy to explore the epithelium of the rat kidney with unsurpassed image quality and detail. In addition, we evaluated diff...
متن کاملEvaluation of variable relative biological effectiveness and the creation of homogenous biological dose in the tumor region in helium ion radiation to the V79 cell line
In radiation therapy, ions heavier than proton have more biological advantages than a proton beam. Recently, ion helium has been considered due to high linear energy transfer (LET) to the medium and a higher relative biological effect (RBE). To design the spread-out Bragg peak (SOBP) of biological dose for radiation with any type of ion, we need exact values of RBE, which is dependent to dose, ...
متن کاملIon Beam Induced Current Measurements of Solar Cells with Helium Ion Microscopy
The scanning electron microscope (SEM) is a versatile high-resolution microscopy tool, and perhaps the most widely used imaging platform across many engineering and scientific fields [1]. Within the last decade, another microscopy technique based on a gaseous field ionization source, utilizing Helium and Neon ions has been introduced [2]. While the popularity of the SEM is hardly challenged by ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2015